Kinetic Control of Multiple Forms of Ca2+ Spikes by Inositol Trisphosphate in Pancreatic Acinar Cells
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منابع مشابه
Kinetic Control of Multiple Forms of Ca2+ Spikes by Inositol Trisphosphate in Pancreatic Acinar Cells
The mechanisms of agonist-induced Ca(2+) spikes have been investigated using a caged inositol 1,4,5-trisphosphate (IP(3)) and a low-affinity Ca(2+) indicator, BTC, in pancreatic acinar cells. Rapid photolysis of caged IP(3) was able to reproduce acetylcholine (ACh)-induced three forms of Ca(2+) spikes: local Ca(2+) spikes and submicromolar (<1 microM) and micromolar (1-15 microM) global Ca(2+) ...
متن کاملTrisphosphate in Pancreatic Acinar Cells
The mechanisms of agonist-induced Ca 2 1 spikes have been investigated using a caged inositol 1,4,5-trisphosphate (IP 3 ) and a low-affinity Ca 2 1 indicator, BTC, in pancreatic acinar cells. Rapid photolysis of caged IP 3 was able to reproduce acetylcholine (ACh)induced three forms of Ca 2 1 spikes: local Ca 2 1 spikes and submicromolar ( , 1 m M) and micromolar (1–15 m M) global Ca 2 1 spikes...
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We have investigated control mechanisms involved in the propagation of agonist-induced Ca2+ waves in isolated mouse pancreatic acinar cells. Using a confocal laser-scanning microscope, we were able to show that maximal stimulation of cells with acetylcholine (ACh, 500 nM) or bombesin (1 nM) caused an initial Ca2+ release of comparable amounts with both agonists at the luminal cell pole. Subsequ...
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We explored a potential structural and functional link between filamentous actin (F-actin) and inositol (1,4,5)-trisphosphate receptors (IP(3)Rs) in mouse pancreatic acinar cells. Using immunocytochemistry, F-actin and type 2 and 3 IP(3)Rs (IP(3)R2 and IP(3)R3) were identified in a cellular compartment immediately beneath the apical plasma membrane. In an effort to demonstrate that IP(3)R distr...
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Inositol 1,4,5-trisphosphate (IP3) induced Ca2+ release in digitonin permeabilized rat pancreatic acinar cells is specifically inhibited by decavanadate. The Ca2+ release induced with 0.18 microM IP3 is half maximally inhibited with approximately 5 microM decavanadate. Complete inhibition is achieved with around 20 microM decavanadate. Removal of decavanadate from the permeabilized cells fully ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1999
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.146.2.405